Fluorescent protein tags ( GFP, RFP, mCherry) are often used for visualization of recombinant proteins in a cellular context and have been used in live cell imaging, reporter assays, Fluorescence Resonance Energy Transfer (FRET), and cell sorting. Peptide tags, such as HA (Hemagglutinin), Myc, DYKDDDDK, and V5 are popularly used for detection in western blot, immunocytochemistry, and co-immunoprecipitation. If the desired downstream application is recombinant protein purification, epitope tags like 6X-His, GST (Glutathione-S-transferase), and MBP (Maltose Binding Protein) are widely used in affinity purification. Epitope Tag Antibodies in Immunoprecipitation and Chromatin ImmunoprecipitationĬhoice of an epitope tag depends on many factors, but downstream application is the most important one.Epitope Tag Antibodies in Immunofluorescence.Overview of Alzheimer’s Disease Antibody Targets.Overview of Parkinson’s Disease Genetics and Related Antibody Targets.Overview of Neural Cell Types and Related Antibody Targets.Overview of Neurobiology Antibody Applications.Overview of Cancer Progression Pathways.An Overview of Pluripotent and Multipotent Stem Cell Targets.An Overview of Immune Checkpoint Research Targets.An Overview of Phosphospecific Antibodies.Secondary Antibody Cross-Adsorption and Cross Reactivity.Antibody Labeling and Immobilization Sites.Comparison of Antibody IgG Binding Proteins.Antibody Isotyping and Characterization Methods.Antibody Production (Immunogen Preparation).Introduction to Antibody Production and Purification.
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